Séminaire Département de Biologie - Dr Sarah CIANFERANI

Thursday, 20th of April 2017 at 11h00
Amphi Curie , Ecole polytechnique

Institut Pluridisciplianire Hubert Curien, UMR 7178
CNRS, University of Strasbourg, France

donnera une conférence intitulée :Advanced Mass Spectrometry for Structural Biology

Structural Mass Spectrometry is a quite recent research area that consists of deriving structural information from MS based data, usually originating from purified proteins or protein complexes. Originally limited to the analysis of the primary structures of proteins, mass spectrometry has evolved over the past 20 years to provide information on the secondary, tertiary and even quaternary structure of proteins.

 The gold standards of structural biology (NMR, crystallography and more recently electron microscopy) are unfortunately sometimes unable to solve the structure of a particular systems for many different reasons, including size, flexibility, heterogeneity of the sample, or the amount of protein needed. All these parameters are, in theory, not a major limitation to most of the MS methods used for secondary and tertiary structure determination. Even though MS-based approaches are rather low-resolution techniques, they represent a potential alternative to get structural information that is complementary to classical biophysical methods.

This presentation will focus on structural mass spectrometry approaches like native mass spectrometry, its hyphenation to ion mobility spectrometry (IM-MS) along with H/D exchange MS and cross-linking approaches. Several case studies will serve to illustrate advantages and drawbacks of the different techniques.

Lieu(x) : Ecole Polytechnique, Amphithéâtre Curie

Contact : Marc Graille